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Data prepared for the conference ESHRE 2016 poster
Genetic Laboratory of the Centre for Reproductive Genetics of iVF Riga Clinic carries out missed abortion material genetic testing basing on a complex analysis of all fetal chromosomes using aCGH method (array Comparative Genomic Hybridization). With the help of this testing may identify unbalanced chromosomal changes in 1-22 pairs of autosomes and in the X, Y sex chromosomes and get a clear answer to the question, whether the cause of an interrupted pregnancy are fetal chromosomal abnormalities.
The first results were obtained by iVF Riga Reproductive Genetics Laboratory
Picture 1: Most common chromosomes found in miscarriage material (in green marked ones found in frame of our study) (Source: M. Aurora, 2007)
Chromosomal aberrations found in missed abortion material
Picture 2: Chromosomal aberrations found in missed abortion material
The data obtained by our clinic also confirm the fact that the most common cause of early pregnancy loss are the changes in the karyotype (set of chromosomes) of the fetus. In material analized in our laboratory more often were found trisomies (the presence of an extra copy of the chromosome) and monosomies (the presence of only one chromosome from the pair), more rarely - polyploidy (more than two complete sets of chromosomes) and structural changes.
STR analysis to exclude DNA contamination
Picture 3: STR analysis to exclude DNA contamination.
In upper picture, red arrows show third STR alleles indicating low level contamination.
In lower picture, no contamination is observed.
(A Short Tandem Repeat (STR) analysis is one of the most useful methods in molecular biology to compare specific loci on DNA from two or more samples. STR analysis measures the exact number of repeating units.)