Preimplantation genetic testing for aneuploidy (PGT-A) is simultaneous testing of the entire set of chromosomes of an embryo on the day 5 of development by NGS method (Next generation sequencing) before the embryo is transferred into the uterus. PGT-A makes it possible to choose the best and most viable genetically competent embryos, thus effectively increasing the rate of implantation, clinical pregnancy and successful birth.<\/p>\n
High risk of the development of aneuploid embryos (with altered chromosome number) in case of parents with normal somatic karyotype:<\/strong><\/p>\n In case of altered karyotype in parents (one or both) or if there is a high risk of passing a genetic disorder to the child:<\/strong><\/p>\n One or both parents are carriers of balanced chromosomal aberrations (Robertsonian or reciprocal translocations) A molecular geneticist together with a clinical geneticist, having received the results of testing by the NGS method, examine them and make a decision on each of the embryos. There are results where the answer is absolutely clear – the embryo is valid or not. However, there are embryos in which mosaic changes have been identified. This means that in the trophectoderm of a particular embryo from which the material was taken, the set of chromosomes was changed only in the part of the cells. And sometimes, if the conclusion about the quality of the embryo is on the border between \u201cvalid for transfer\u201d and \u201cnot valid for transfer\u201d, only the experience and knowledge of molecular geneticist, geneticist and reproductologist allow us to make a joint decision on the suitability of such an embryo for medical fertilization.<\/p>\n We are the only clinic in Latvia who performs Preimplantation genetic testing on site, in our own laboratory. The iVF Riga Genetic centre began his work in 2014, and already in 2015, the first child was born after PGT-A.<\/strong> Much is being talked about is it necessary to make PGT-A or not? But the main question is: does this method really help \u2013 and for whom? What really is PGT-A? Preimplantation genetic testing for aneuploidy (PGT-A) is simultaneous testing of the entire set of chromosomes of an embryo on the day 5 of development by NGS method (Next generation sequencing) before the embryo is transferred into the uterus. PGT-A makes it possible to choose the best and most viable genetically competent <\/p>\n\n
\nMale infertility factor associated with AZF microdeletions (Y-chromosome)
\nOne or both parents are carriers of X-chromosome mutation (risk of development of e. g. Duchenne\/Becker muscular dystrophy, fragile X chromosome syndrome, haemophilia and other disorders)
\nPGT-A \u2013 step by step
\nIVF\/ICSI manipulation
\nCultivation of the received embryos up to day 5 (blastocyst)
\nEmbryo biopsy \u2013 several trophectoderm (outer layer) cell collection without harming the embryo and without any damage to its further development – to determine whether this embryo is healthy and suitable for transfer
\nEmbryo freezing (vitrification) for later use
\nPGS \u2013 Preimplantation Genetic Screening \u2013 Chromosomal analysis
\nSelection of genetically competent embryos for embryo transfer
\nEmbryo transfer<\/p>\nHow to get the answers?<\/h4>\n
Who carries out this testing in Latvia?<\/h4>\n
\nWe perform PGT-A and PGT-M testing for other Latvian and foreign clinics.<\/strong><\/p>\nConclusion<\/h4>\n
\nAt the moment, we have tested 2616 embryos. On average, we test 100 embryos per month, and from our own experience, we see that PGT-A testing improves the success rate of IVF cycles. Therefore, we recommend this method to save you from unnecessary worries and help to achieve the desired results as soon as possible.<\/p>\nSo, PGT-A is carried out:<\/h4>\n
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Three conditions are required for the complete and effective use of this method:<\/h4>\n
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